Nitric oxide levels in cochlear fluids of guinea pig.

نویسندگان

  • Fernando Kaoru Yonamine
  • Ektor Tsuneo Onishi
  • Margareth Gori Mouro
  • Elisa Mieko Suemitsu Higa
  • Oswaldo Laércio Mendonça Cruz
چکیده

PURPOSE To describe a technique to obtain guinea pigs cochlear fluids and measure nitric oxide (NO) concentration. METHODS Six guinea pigs were used and sacrificed. The cochlear fluids collected for measurement of NO, performed by chemiluminescence (NOA 280). RESULTS Through the chemiluminescence was possible to analyze the concentration of NO in cochlear fluids obtained. Average levels of nitric oxide from guinea pigs was 12.55 µM. CONCLUSION It is possible to obtain nitric oxide cochlear fluids, with this technique and nitric oxide concentration measure by chemiluminescence, a quantitative and more precise method.

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عنوان ژورنال:
  • Acta cirurgica brasileira

دوره 28 5  شماره 

صفحات  -

تاریخ انتشار 2013